High-throughput screening of soluble recombinant proteins
نویسندگان
چکیده
منابع مشابه
High-throughput screening of soluble recombinant proteins.
The aims of high-throughput (HTP) protein production systems are to obtain well-expressed and highly soluble proteins, which are preferred candidates for use in structure-function studies. Here, we describe the development of an efficient and inexpensive method for parallel cloning, induction, and cell lysis to produce multiple fusion proteins in Escherichia coli using a 96-well format. Molecul...
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The aim of this work was to investigate and determine central parameters that can be used to control and increase the solubility, quality and productivity of recombinant proteins. These central parameters should be applicable under the constraints of high-throughput protein production in Escherichia coli. The present investigation shows that alternative methods exist to improve solubility, qual...
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The recombinant expression of integral membrane proteins is considered a major challenge, and together with the crystallization step, the major hurdle toward routine structure determination of membrane proteins. Basic methodologies for high-throughput (HTP) expression optimization of soluble proteins have recently emerged, providing statistically significant success rates for producing such pro...
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within autosomal genes are unavailable for assay validation. However, male samples are equivalent to a heterozygous deletion in female samples. Consequently, female samples with heterozygous deletions of an X chromosomal segment are comparable to normal male samples. RD-PCR was also tested in a prospective study in hemophilia A families to detect heterozygous deletions in X chromosomal segments...
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ژورنال
عنوان ژورنال: Protein Science
سال: 2009
ISSN: 0961-8368
DOI: 10.1110/ps.0205202